12 well loading control western blot software#

The ChemiDoc MP Imaging System is a full-feature, best-in-class instrument for imaging and quantifying nucleic acid and protein in gels and western blots.

The ChemiDoc Imaging System allows sensitive imaging and documentation of DNA/protein gels and chemiluminescent western blots Order Now Trans-Blot Turbo Transfer System #1704150īlotting instrument, includes base, 2 cassettes to hold 1–2 midi or up to 4 mini blotting sandwiches, blot roller Order Now.Vertical midi-format electrophoresis cell, includes buffer tank, lid with power cables, 3 sample loading guides (12+2-well, 18-well, and 26-well) Order Now Mini-PROTEAN Tetra Vertical Electrophoresis Cell for Mini Precast Gels, 4-gel #1658004Ĥ-gel vertical electrophoresis system, includes electrode assembly, companion running module, tank, lid with power cables, mini cell buffer dam Order Now.Stain-Free technology noticeably lowers the background signal in comparison to traditional total protein detection methods. Stain-Free technology allows scientists to visualize strong total protein signals with low background on their gels and blots that would otherwise not be possible without additional staining steps thus, improving overall dynamic range without the need to perform extra staining and destaining steps.Ĭomparison between high background and low background signal in gels. The design of a quantitative western blot experiment. Although the actin and tubulin signals appear linear, the densitometric ratio was far below the predicted “quantitative response” of actual loading whereas the stain-free signal correlated to the expected result (e). Lane labels correspond to total protein load (μg). Linearity comparison of stain-free total protein measurement and immunodetection of three housekeeping proteins in 10–50 μg of HeLa cell lysate. On the left are representative images of (a), stain-free blot and the chemi blots for (b), β-actin (c), β-tubulin and (d), GAPDH. Total Protein normalization using Stain-Free technology consistently delivers scalar linear response across a wider dynamic range. The unique characteristics of Stain-Free technology makes imaging of total protein a much better method for quantitative western blotting.įollowing a brief UV light activation, Stain-Free ﬂuorochromes are covalently bound to protein molecules in the gel, allowing them to be imaged repeatedly on the gel or on a membrane post-transfer. The use of traditional total protein stains can hinder downstream visualization of a western blot, usually requiring repetitive staining and detaining steps which is one of the obstacles preventing wider adaptation of TPN. Why is Stain-Free Total Protein Normalization Superior over Other Methods? Accounts for variations during loading, electrophoresis, and transfer.Eliminates immuno-blotting interference associated with HKP.Enables total protein in load sample to be measured.

Total protein normalization using Stain-Free technology This technology makes it easy for researchers to adopt total protein loading control for western blotting. Stain-Free blot visualization and image acquisition require only a few seconds using a stain-free enabled imager. Stain-Free labeled proteins can be visualized in gels 1 minute after gel electrophoresis.

12 well loading control western blot software#

An illustration of the total protein normalization feature in the Image Lab Software using a Stain-Free enabled imager.īio-Rad’s Stain-Free western blotting provides a practical, convenient, and reliable way to perform total protein normalization.